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Reconstitution of the t 6 A modification was carried out at 37 C for 1.5 h in a reaction mixture containing 100 mM HEPESKOH (pH 7.6), 25 mM MgCl 2 , 25 mM KCl, 5 mM DTT, 2 mM ATP, 10 mM NaHCO 3 , 5 mM l-threonine, 2.5 M transcribed tRNA and 2.5 mM each E

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